Mechanisms of Endothelial Differentiation in Embryonic Vasculogenesis

Vasculogenesis: Differentiation of Endothelial Cells and Vascular Plexus Formation

The formation of the circulatory system precedes that of all other organ systems during vertebrate development, born out of a need to ensure rapid delivery of nutrients and oxygen to tissues of the growing embryo. Thus, as the developing organism continues to increase in size and undergo cellular differentiation and morphogenesis, the vasculature likewise expands, remodels, and becomes speciali...

Endothelial differentiation of embryonic stem cells.

Vascular progenitor cells derived from stem cells could potentially lead to a variety of clinically relevant applications, including cell-based therapies and tissue engineering. Here, we describe methods for isolating purified proliferating populations of vascular endothelial cells from mouse embryonic stem cells (mESC) using Flk-1 positive sorted cells, VEGF supplementation, and a rigorous man...

differentiation of murine embryonic stem cells into endothelial cells

objective: in this investigation murine embryonic stem (es) cells were differentiated into endothelial cells. materials and methods: murine es cells (cce cell line) exposed to alpha-mem medium containing 10% fbs for 4 days. then obtained flk-1 (flk-1: vascular endothelial growth factor receptor 2) positive cells were cultuted in endothelial growth medium-2 (egm-2) until the last day of experime...

Endothelial Differentiation of Embryonic Stem Cells

Maturation of embryonic stem cells into endothelial cells in an in vitro model of vasculogenesis.

A primitive vascular plexus is formed through coordinated regulation of differentiation, proliferation, migration, and cell-cell adhesion of endothelial cell (EC) progenitors. In this study, a culture system was devised to investigate the behavior of purified EC progenitors in vitro. Because Flk-1(+) cells derived from ES cells did not initially express other EC markers, they were sorted and us...